Optimizing Tear Collection in Mice for mRNA and Protein AnalysisMarysol Bello 1, Andrea Morales-Farfán 1, Erick J. Martínez-Colín 1, Ivonne Lezama 1, Monica Lamas 1
1Departamento de Farmacobiología, Centro de Investigación sobre el Envejecimiento, CINVESTAV Sede Sur
The identification of RNA and protein biomarkers from tears in mouse models holds great promise for early diagnostics in various diseases. This manuscript provides a comprehensive protocol for optimizing the efficacy and efficiency of mRNA and protein isolation from mouse tears.